CD45RO (CD45 Antigen, B220, GP180, Leukocyte Common Antigen, LCA, L-CA, LY5, LY-5, Protein Tyrosine Phosphatase Receptor Type C Polypeptide, PTPRC, T200, T200 Glycoprotein), Clone: [UCHL1], Mouse, Monoclonal

Produktübersicht

• Artikelname: CD45RO (CD45 Antigen, B220, GP180, Leukocyte Common Antigen, LCA, L-CA, LY5, LY-5, Protein Tyrosine Phosphatase Receptor Type C Polypeptide, PTPRC, T200, T200 Glycoprotein), Clone: [UCHL1], Mouse, Monoclonal
• Artikelnummer: USB-C2400-67G
• Hersteller Artikelnummer: C2400-67G
• Alternativnummer: USB-C2400-67G-100
• Hersteller: US Biological
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: FC, IHC, IP, WB
• Immunogen: IL-2 dependent T cell line, CA1

Produktbeschreibung

CD45RO is a 180 kD single chain membrane glycoprotein. It is a splice variant of the tyrosine phosphatase CD45, lacking the A, B, and C determinants. The CD45RO isoform is expressed on activated and memory T cells, some B cell subsets, activated monocytes/macrophages, and granulocytes. CD45RO enhances both T cell receptor and B cell receptor signaling mediated activation. CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1 and CD22. CD45 isoform expression can change in response to cytokines. The UCHL1 antibody is commonly used in combination with antibodies against CD45RA to discern memory and naive T cells. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [UCHL1]
• Reinheit: Purified by affinity chromatography.
• Formulierung: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.