CD49d (Antigen CD49d, CD49d Antigen, CDw49d, Alpha 4 Subunit of VLA-4 Receptor, Integrin alpha IV, Integrin alpha 4, IA4, ITGA4, LPAM23, MGC90518, Very Late Activation Protein 4 Receptor Alpha 4 Subunit, VLA4, VLA-4) (MaxLight 550
Artikelnummer:
USB-C2404-46G2-ML550
Hersteller Artikelnummer:
C2404-46G2-ML550
Alternativnummer:
USB-C2404-46G2-ML550-100
Hersteller:
US Biological
Wirt:
Mouse
Kategorie:
Antikörper
Applikation:
FC, IHC
Immunogen:
HOON pre-B leukemia cell line
MaxLight(TM)550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor(TM)546, 555, DyLight(TM)549 , Cy3(TM), TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm), Emission (575nm), Extinction Coefficient 150,000. CD49d (VLA-4) inhibits adhesion of rat lymphocytes to cytokine-activated endothelial cells. It blocks binding of rat lymphocytes to purified VCAM-1 and to the CS-1 peptide of fibronectin. VLA-4 is a leukocyte integrin which plays an important role in lymphocyte adhesion and activation. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 10e6 cells in 100ul Immunohistochemistry (Frozen): Overnight incubation with C2404-46G2 and acetone fixation for frozen sections is recommended. Not suitable for use with paraffin sections. Optimal dilutions to be determined by the researcher. Hybridoma: SP2.0-Ag14 myeloma cells with spleen cells from Balb/c mice Positive Control for Immunohistochemistry: Human tonsil Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight(TM)550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
