CD49d is a ~150kD single pass type I membrane glycoprotein that can associate with either beta 1 integrin (CD29) or beta 7 integrin to form heterodimers CD49d/CD29 (VLA-4) and alpha4/beta7 (LPAM-1) respectively (Holzmann et al. 1989). CD49d is expressed on most lymphocytes, granulocytes, monocytes and thymocytes. The primary ligands for CD49d are CD106 (VCAM-1), fibronectin and MAdCAM-1 (Sheppard et al. 1994). This clone has also been reported to block the binding of CD49d to its ligands (Andrew et al. 1994). Applications: Suitable for use in Flow Cytometry. Other applications have not been tested. Recommended Dilution: Flow Cytometry: Neat. Use 10ul to label 1x10e6 cells in 100ul. Optimal working dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Fisher rats. Storage and Stability: Store product at 4C before and after reconstitution. DO NOT FREEZE! Lyophilized and reconstituted products are stable for 12 months at 4C after receipt. Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product centrifuge the original vial prior to removing the cap.
Purified by Protein G affinity chromatography from tissue culture supernatant.
Formulierung:
Supplied as a lyophilized powder in PBS, 0.1% BSA, 5% sucrose, 10mM sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml sterile ddH2O.
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