VLA-4 belongs to the beta1-integrin family, which functions both as cell-matrix and cell-cell receptors (1-3). As a matrix receptor VLA-4 binds to an alternatively spliced domain of fibronectin, while as a cellular receptor it associates with its counter-receptor ligand VCAM-1 (1,2). In addition to CD44/pgp-1, VLA-4 has been shown to be important for lymphopoiesis, and is also believed to be important for extravasation of lymphocytes into sites of inflammation as a second signal in addition to LFA/ICAM-1 (2,3). CD49d/VLA-4 is expressed in low amounts on resting lymphocytes and monocytes, is absent or minimally detectable on stromal cells, whereas virtually all lymphoid and myeloid cells in bone marrow and in long-term culture are positive (1,2). Applications: Identification and enumeration of CD49d+ cells by flow cytometry 1, 2 Studies of cell-cell interaction (1, 2) and tumor cell metastasis and adhesion 4 Immunohistochemical studies 3 Inhibition of lymphopoiesis in Whitlock-Witte long-term bone marrow cultures 1 Retardation of myelopoiesis in Dexter-type cultures 1 Immunoprecipitation 1 Recommended Dilution: Flow Cytometry: 2ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20C or colder. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Klonalität:
Monoclonal
Klon-Bezeichnung:
[PS/2]
Reinheit:
Purified
Formulierung:
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. Labeled with Biotin.
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