VLA-4 belongs to the beta1-integrin family, which functions both as cell-matrix and cell-cell receptors (1-3). As a matrix receptor VLA-4 binds to an alternatively spliced domain of fibronectin, while as a cellular receptor it associates with its counter-receptor ligand VCAM-1 (1,2). In addition to CD44/pgp-1, VLA-4 has been shown to be important for lymphopoiesis, and is also believed to be important for extravasation of lymphocytes into sites of inflammation as a second signal in addition to LFA/ICAM-1 (2,3). CD49d/VLA-4 is expressed in low amounts on resting lymphocytes and monocytes, is absent or minimally detectable on stromal cells, whereas virtually all lymphoid and myeloid cells in bone marrow and in long-term culture are positive (1,2). Applications: Identification and enumeration of CD49d+ cells by flow cytometry 1, 2 Studies of cell-cell interaction (1, 2) and tumor cell metastasis and adhesion 4 Immunohistochemical studies 3 Inhibition of lymphopoiesis in Whitlock-Witte long-term bone marrow cultures 1 Retardation of myelopoiesis in Dexter-type cultures 1 Immunoprecipitation 1 Recommended Dilution: Flow Cytometry: 0.2ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Klonalität:
Monoclonal
Klon-Bezeichnung:
[PS/2]
Reinheit:
Purified
Formulierung:
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide and a stabilizing agent. Labeled with R-Phycoerythrin (PE).
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