CD49e (CD49 Antigen-like Family Member E, Fibronectin Receptor alpha, FNRA, Integrin alpha-F, Integrin alpha 5, Integrin alpha-5, ITGA5, Very Late Activation Protein 5 alpha, VLA-5, VLA5A), IgG2a, Clone: [11n34], Mouse, Monoclonal
Artikelnummer:
USB-C2405-02L1
Hersteller Artikelnummer:
C2405-02L1
Alternativnummer:
USB-C2405-02L1-100
Hersteller:
US Biological
Wirt:
Mouse
Kategorie:
Antikörper
Applikation:
WB
Immunogen:
Recombinant protein corresponding to Phe42-Tyr995 from human CD49e, expressed in CHO cells.
Integrin a5, is a 140-150kD integrin alpha chain that forms dimers with at least five beta chains including b1, 3, 5, 6, and 8. It is a 1018aa residue type I membrane protein with a large 962aa extracellular domain (ECD) and a short 32aa cytoplasmic tail. The N-terminal region of Integrin a5, which is important for ligand binding, contains seven FG-GAP (phenylalanyl-glycyl and glycyl-alanyl-prolyl) consensus repeats that fold into a b-propeller domain. Furin cleavage of the Integrin a5 ECD occurs after Gly 889, generating a disulfide-linked, heteromeric subunit Integrin a5 chain. Integrin a5-containing integrins bind multiple ECM molecules, including Vitronectin, Osteopontin, MMP-2, and TSP. The ECD of human Integrin 5 shares 92aa sequence identity with mouse Integrin a5 ECD. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml using K562 human chronic myelogenous leukemia cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
