Human CD49f molecules are integrin alpha 6 chains that are expressed as heterodimers with CD29 (beta1integrin) or CD104 (beta4 integrin) and function as adhesion receptors. CD49f is expressed on platelets, monocytes, T lymphocytes and thymoctyes. The ligands for CD49f/CD29 include laminin and invasion. CD49f/CD29 has been shown to associate with CD63. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ug/ml Immunohistochemistry: frozen Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. 5 x 10e5 cultured Raji human tumor cells were washed and incubated for 45 minutes on ice with 80ul of C2405-04N at 10ug/ml. 2. Cells were washed twice and incubated with I1904-06F. 3. Cells were washed three times, fixed and analyzed by FACS. 4. Cells stained positive with a mean shift of 0.78 log10 fluorescent units when compared to a mouse IgG1 negative control (I1904-78) a similar concentration. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Klonalität:
Monoclonal
Klon-Bezeichnung:
[7H164]
Isotyp:
IgG1
Reinheit:
Purified by Protein A affinity chromatography.
Formulierung:
Supplied as a liquid in PBS, pH 7.5, 0.5mg/ml gentamicin sulfate.
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