Cell suspension containing osteoclasts from osteoclastomas
CD51 represents the 150kD integrin alphaV chain that associates with integrin beta3 (CD61) to form the CD51/CD61 vitronectin receptor on endothelial cells, certain activated leukocytes, NK cells, macrophages, neutrophils and platelets. CD51 is the most promiscuous integrin a subunit, it can form heterodimers with the beta1 (CD29), beta3 (CD61), beta5, beta6 and beta8 subunits in various tissues. CD51/CD61 acts as an activation-independent receptor for platelet attachment and spreading on vitronectin and other RGD-containing proteins, including matrix components. It also mediates leukocyte-endothelial cell adhesion via interaction with CD31.1-4 Applications: Suitable for use in FLISA, Immunohistochemistry, Immunoprecipitation, Flow Cytometry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: frozen Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
