Applications: Suitable for use in Immunohistochemistry, Immunocytochemistry, Immunoprecipitation, Flow Cytometry and FLISA. Other applications not tested. Recommended Dilutions: Immunohistochemistry: For use on acetone or paraformaldehyde fixed tissue Immunocytochemistry: Will react with some lymphoid cell lines (B cells), many carcinoma and melanoma cell lines and osteosarcomas. Optimal dilution determined by the researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.