The CD52 antigen is also known as CAMPATH 1. It is a remarkably small peptide that is heavily glycosylated. It is attached to the cell surface membrane via a GPI link. The apparent molecular mass of the antigen on SDS-PAGE is 25-29kD. CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable. Applications: Suitable for use in Cytotoxicity Assays, ELISA, Flow Cytometry, Immunohistochemistry (frozen, resin, and paraffin), Immunoprecipitation, and Western Blot. Other applications not tested. Recommended Dilution: Flow cytometry: 1:50-1:100. Use 10ul of the suggested working dilution to label 1x10e6 cells in 100ul. Cytotoxic Assays: 50ug/ml (Use human serum as complement source) Optimal dilutions to be determined by the researcher. Recommended Positive Control Tissue: Tonsil Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
