The antibody is the prototypic antibody for the CD59 determinant, a PI-linked glycoprotein of 18-20kD, which is broadly expressed on most types of of cells. The antigen is the major complement-protecting molecule of human cells. It is expressed on all leukocytes, erythrocytes and most other cell types. Binding of this antibody inhibits the complement-protecting activity of CD59. CD59 is one of the complement regulatory membrane proteins that is missing in Paroxysmal Nocturnal Haemoglobinuria (PNH). Anti CD59 reagents may therefore be useful in the differential diagnosis of PNH type II and PNH type I as reported. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 20ul labels human blood cells in 100ul whole blood. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
