CD61 associates with either the alpha IIb integrin (CD41) or the alpha V integrin (CD51) at the cell surface. CD61 is expressed on platelets and megakaryocytes in association with CD41, and on endothelial cells, monocytes, platelets and osteoclasts in association with CD51. CD61 is a receptor for fibrinogen, fibronectin, vWF, vitronectin and thrombospondin. Applications: Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:100-1:200 Use 10ul to label 1x10e6 cells in 100ul. Immunohistochemistry: Frozen sections Immunohistochemistry (Paraffin): Requires protein digestion pre-treatment of paraffin sectons (e.g. trypsin prior to staining) Optimal dilutions to be determined by the researcher. Hybridoma: Spleen cells from immunized Balb/c mice were fused with cells of the NS1 myeloma cell line. Recommended Secondary Antibodies : I1904-65W: IgG, F(ab)2 (HRP) Pab Rb xMo I1904-66C: IgG, F(ab)2X-adsorbed (HRP) Pab Rb xMo I1904-66A: IgG, F(ab)2 (FITC) Pab Rb xMo Recommended Negative Control: I1904-78R: IgG1 Murine Negative Control Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
