Phorbol myristic acid activated human peripheral blood leukocytes.
CD62L is a 74-95 kD single chain type I glycoprotein referred to as L-selectin or LECAM-1. It is expressed on most peripheral blood B cells, subsets of T and NK cells, monocytes, granulocytes, and certain hematopoietic malignant cells. CD62L binds to carbohydrates present on certain glycoforms of CD34, glycam-1, and MAdCAM-1 and with a low affinity to anionic oligosaccharide sequences related to sialylated Lewis x (sLex, CD15s) through its C-type lectin domain. CD62L is important for the homing of naive lymphocytes to high endothelial venules in peripheral lymph nodes and Peyers patches. It also plays a role in leukocyte rolling on activated endothelial cells. Applications: Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 0.5ug per 10e6 cells in 100ul or 100ul whole blood. Immunohistochemistry: Reported to stain acetone-fixed frozen tissue sections. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
