Human CD62E (E-selectin) is an adhesion molecule expressed on activated endothelium and facilitates recruitment of leukocytes to sights of acute inflammation and immunological events. CD62E has affinity to LewisX (1). Applications: Suitable for use in Flow Cytometry and blocking the function of CD62E. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 (80ul/test) Optimal dilutions to be determined by the researcher. Performance: 1. Cultured human umbilical cord vein endothelialcells (HUVEC) were stimulated 4 hours with 10 ng/ml Phorbol 12-Myristate 13-Acetate. 2. Five x 10e5 cells per tube were harvested by ETDA method, washed and incubated 45 minutes on ice with 80 ul of C2414-94F at a 1:50 dilution factor (20ug/ml). 3. They were then washed 3 times, fixed and analyzed on a BD FACS Calibur. 4. A net 64% population of the cells stained positive with a mean shift of 0.84 log10 fluorescent units when compared to a Mouse IgG1/FITC negative control at a similar concentration. 5. Binding was 75% blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml C2414-94D. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Klonalität:
Monoclonal
Klon-Bezeichnung:
[7H188 HAE-1f]
Isotyp:
IgG1
Reinheit:
Purified by Protein A affinity chromatography.
Formulierung:
Supplied as a liquid in PBS, pH 7.5, 5% glycerol, 0.2% BSA, 0.04% sodium azide. Labeled with FITC. Flourescein/IgG Molar Ratio: 6.7
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