CD62L, also known as L-selectin and LECAM-1, is a 74-94kD type I glycoprotein expressed on neutrophils, monocytes, T and B cell subsets and NK cells. A soluble form of CD62L is present at high levels in blood. Like other selectins, CD62L mediates the initial attachment and rolling of leukocytes on endothelial surfaces which is the prerequisite for leukocyte extravasation from the blood into tissues. More specifically, CD62L is important for the homing of naive lymphocytes via high endothelial venules to peripheral lymph nodes and Peyers patches. It also contributes to the recruitment of leukocytes to areas of inflammation. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: neat, 10ul labels 10e6 cells in 100ul. Optimal dilutions to be determined by the researcher. Positive Control: Human tonsil Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
