CD62P is expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leukocytes and endothelial cells. Applications: Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications have not been tested. Recommended Dilutions: Optimal dilution to be determined by researcher. Hybridoma: Spleen cells from immunized CD62P knock-out mice (strain C57/B6) were fused with cells of the NS-1 myeloma cell line. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
