CD80 (B7-1) is a 60kD cell surface glycoprotein which is a member of the CD28/B7 family. In mice, CD80 is expressed on monocytes, peritoneal macrophages and dendritic cells, and expression may be significantly increased upon B lymphocytes by LPS and by IL-4. CD80 has been identified as a ligand for CD28 and cytotoxic T-lymphocyte antigen-4 (CTLA-4), two structurally similar molecules expressed on T cells. CD28 and CTLA4 are two receptors that have essential but opposing functions in T-cell stimulation. The Interaction of CD80 with CD28 stimulates and sustains T cell responses, whereas the interaction of CD80 with CTLA4 is reported to inhibit T-cell responses. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:10-1:50, 10ul labels 10e6 cells in 100ul Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Hybridoma: P3U1 myeloma cells with spleen cells from SD rats. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
Klonalität:
Monoclonal
Klon-Bezeichnung:
[10B2050 RM80]
Isotyp:
IgG2a
Reinheit:
Purified by Protein G affinity chromatography from tissue culture supernatant.
Formulierung:
Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
