Rat myeloma YB2/0 transfected with truncated mouse IL-2RB cDNA.
The IL-2 receptor is a complex of three distinct polypeptide chains: (i) the alpha chain which binds IL-2 with low affinity, (ii) the beta chain that binds IL-2 with high affinity, and (ii) the common gamma chain (gC) that does not bind IL-2.2 The high affinity receptor complex is an alpha/beta/gamma heterotrimer with a Kd of 1.3 x 10-11 M.2 In mouse spleen, CD122 is expresed on ~ 30% of CD8+ cells and all NK cells, but < 1% of B cells and CD4+ T lymphocytes. In the thymus, its expression is confined to CD4-CD8+ single positive and CD4-CD8-double negative cells.4 Cytoplasmic regions of the IL-2R beta chain are involved in IL-2-mediated cellular signaling and, via the interaction of IL-2 and its receptor complex, may be involved in the generation and differentiation of T lymphocytes.1, 4 Applications: Flow cytometry 1 Immunoprecipitation 1 Recommended Dilution: Flow Cytometry: 1ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20C or colder. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Klonalität:
Monoclonal
Klon-Bezeichnung:
[5H4]
Reinheit:
Purified.
Formulierung:
Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with Biotin.
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