CD152 Human, IgG2a Mouse Fc, Fusion Protein (CTLA4, Cytolytic T-lymphocyte-associated Antigen 4) (PE)

Produktübersicht

• Artikelname: CD152 Human, IgG2a Mouse Fc, Fusion Protein (CTLA4, Cytolytic T-lymphocyte-associated Antigen 4) (PE)
• Artikelnummer: USB-C2548-83V3
• Hersteller Artikelnummer: C2548-83V3
• Alternativnummer: USB-C2548-83V3-50
• Hersteller: US Biological
• Kategorie: Molekularbiologie
• Applikation: FC, FLISA

Produktbeschreibung

Immune response mediated by T cells can be characterized to functionally proceed as follows: antigen recognition by the T cell receptor, activation through costimulation, effector activities to eliminate antigen and finally down regulation. Human CD152 is a cell surface glycoprotein expressed at low levels on activated T cells. CD152 is a high affinity receptor for the costimulatory molecules CD80 (B7-1) and CD86 (B7-2) and appears to function as a negative regulator of T cell activation. Therefore, CD152 may be an important player in down regulating T cell mediated immune responses. The CD152 Ig fusion protein has biological activity and binds with high affinity to human or mouse CD80 (B7-1) and CD86 (B7-2). CD152 Ig will block the binding of anti-CD80 (B7-1) and anti-CD86 (B7-2) monoclonal antibodies. Functional Application: CD152 Ig binds with high affinity to human or mouse CD80 (B7-1) and CD86 (B7-2)(4, 5). CD152 Ig blocks the binding of anti-CD80 (B7-1) and anti-CD86 (B7-2) mAbs. Human CD152(CTLA-4)-muIg/R-PE Fusion Protein (also binds to mouse CD80/CD86) Molecular Structure: A soluble 110 kd dimeric fusion protein consisting of the extracellular (125aa) domain of human CD152 (CTLA-4) fused to murine IgG2a Fc (233aa). Transfectant Cell Line: BHK Applications: Suitable for use in FLISA and Flow Cytometry. Other applications not tested. Recommended Dilution Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 6 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Produkteigenschaften

• Molekulargewicht: 40.1
• Reinheit: Purified by Protein A affinity chromatography. 95% by SDS-PAGE
• Formulierung: Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 500mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 15% glycerol. Labeled with R-Phycoerythrin (PE).