CD40 is a member of the TNF receptor family. it is a cell surface protein expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells. Signaling though CD40 plays an important role in the proliferation and differentiation of B cells, and is critical for immunoglobulin (Ig) class switching. The membrane-anchored CD40-Ligand is expressed almost exclusively on activated CD4+ T lymphocytes. Failure to express CD40L leads to ?immunodeficiency with hyper-IgM, a disease characterized by failure to produce IgG, IgA and IgE. The human CD40L gene codes for a 261aa type II transmembrane protein, which contains a 22aa cytoplasmic domain, a 24aa transmembrane domain and a 215aa extracellular domain. The soluble form of CD40L is an 18kD protein comprising the entire TNF homologous region of CD40L. It is generated in vivo by an intracellular proteolytic processing of the full length CD40L. Recombinant human soluble CD40 ligand is a 16.3kD protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CD40L. Recombinant protein corresponding to human CD154, expressed in E. coli Amino Acid Sequence: MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Manufactured using all Animal-Free reagents.
98% by SDS-PAGE gel and HPLC analyses. Endotoxin: 0.01ng/ug (<0.1EU/ug)
Formulierung:
Supplied as a lyophilized powder in 10mM sodium phosphate, pH 7.5. Reconstitute in sterile ddH2O to 0.1-1mg/ml, which can then be further diluted to other aqueous solutions.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
