Cfr9I (SmaI)

Produktübersicht

• Artikelname: Cfr9I (SmaI)
• Artikelnummer: USB-C3250
• Hersteller Artikelnummer: C3250
• Alternativnummer: USB-C3250-300,USB-C3250-1500
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

5-C C C G G G-3 3-G G G C C C-5 Unlike SmaI, Cfr9I produces DNA fragments with a 4-base 5-extension. To achieve complete digestion of substrate with Cfr9I, the concentration of DNA should not be less than 50ug/ml in a reaction buffer. Concentration: 10u/ul Source: E.coli that carries the cloned cfr9IR gene from Citrobacter freundii RFL9 Buffer: 10mM Tris-HCl (pH 7.2), 5mM MgCl2, 200mM sodium glutamate and 0.1mg/ml BSA. Incubate at 37C Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. For longer periods-the Storage Buffer should be used. Storage Buffer: 10mM Tris-HCl (pH 7.5 at 25C), 250mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol.