| Non-denaturing zwitterionic detergent with cholic acid and sulfobetaine moieties, used to dissolve biological membranes, useful for protein extraction methods due to its ease of removal by dialysis or gel filtration. Low background UV absorption for protein detection. Designed by Good, et al., and typically referred to as Goods buffer useful in cell culture media formulations. Selection of biological buffer systems should include the following criteria: exclusion by biological membranes, low absorption between 240 and 700nm, chemically stable, and stable to temperature and concentration changes. Synonyms: (3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate), N,N-Dimethyl-N-(3-sulfopropyl)-3-[[(3alpha,5beta,7alpha,12alpha)-3,7,12-trihydroxy- 24-oxocholan-24-yl]amino]-1-propanaminium Inner Salt, CHAPS CAS Number: 75621-03-3 Molecular Formula: C32H58N2O7S Molecular Weight: 614.88 Appearance: Supplied as a white to off white powder. Purity: 98% (typically 99%) Melting Point: 255-260C Solubility (10% aq): Colorless, clear, complete. Soluble in DMF, hot Methanol, Water (50mg/1ml) Loss on Drying: 4.0% TLC: One spot Storage and Stability: RT short-term, -20C long-term. Hygroscopic. Keep tightly closed and desiccated. |
