Carboxyl ester lipase, also known as bile saltstimulated lipase, belongs to the TypeB carboxyl esterase/lipase family of enzymes. The enzyme possesses a SerHisAsp catalytictriad, and is capable of hydrolyzing both ester and amide bonds. CEL is a nonspecificlipase which hydrolyzes a variety of substrates, including cholesterylesters, acylglycerols, and ceramide (1) . CEL is highly expressed by pancreatic acinar cells and secreted into the gastrointestinal tract, where it contributes to the digestion of dietary lipids (2). It is expressed at much lower levels by the liver, macrophages, and endothelial cells (3). CEL is present in the circulation, where it may play a role in low densi ty lipoprotein metabolism and atherogenesis (4). Source: Murine myeloma cell line, NS0-derived Ala21 Leu534, with a C-terminal 6 His tag, Accession Q64285. Endotoxin: 1.0 EU per 1ug of the protein by the LAL method. Activity: Measured by its ability to cleave p-Nitrophenylbutyrate (PNPB). The specific activity is 4,000 pmol/min/ug, as measured under the described conditions. See Activity Assay Protocol. Specific Activity (pmoles/min/ug) = Adjusted Abs* (OD) x Conversion Factor** (pmole/OD) Incubation time (min) x amount of enzyme (ug) Storage and Stability: 12 months from date of receipt, 20 to 70C as supplied. 1 month, 2 to 8C under sterile conditions after reconstitution. 3 months, 20 to 70C under sterile conditions after reconstitution. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
