Cholinergic Receptor, Nicotinic, alpha 7, Exons 5-10 (CHRFAM7A, CHRNA7 and FAM7A Family with Sequence Similarity 7A, Exons A-E Fusion, CHRNA7, CHRNA7-DR1, CHRNA7-FAM7A Fusion Protein, D-10, MGC120482, MGC120483), Goat
Artikelnummer:
USB-C5062-74
Hersteller Artikelnummer:
C5062-74
Alternativnummer:
USB-C5062-74-100
Hersteller:
US Biological
Wirt:
Goat
Kategorie:
Antikörper
Applikation:
ELISA
Immunogen:
Synthetic peptide, QKYCIYQHFQFQ, from the N-Terminus of human CHRFAM7A.
The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The family member CHRNA7, which is located on chromosome 15 in a region associated with several neuropsychiatric disorders, is partially duplicated and forms a hybrid with a novel gene from the family with sequence similarity 7 (FAM7A). Alternative splicing has been observed, and two variants exist, for this hybrid gene. The N-terminally truncated products predicted by the largest open reading frames for each variant would lack the majority of the neurotransmitter-gated ion-channel ligand binding domain but retain the transmembrane region that forms the ion channel. Although current evidence supports transcription of this hybrid gene, translation of the nicotinic acetylcholine receptor-like protein-encoding open reading frames has not been confirmed. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA:1:1000 Optimal dilutions to be determined by the researcher. Positive Control: Human Brain (Amygdala, Hippocampus, Cerebellum) lysates Storage and Stability: May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 monthsat -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See C5062-74-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
