CHOP (C/EBP Homology Protein, GADD153, Growth Arrest and DNA Damage Inducible Protein 153), Clone: [9C8], Mouse, Monoclonal
Artikelnummer:
USB-C5064-05A
Hersteller Artikelnummer:
C5064-05A
Alternativnummer:
USB-C5064-05A-100
Hersteller:
US Biological
Wirt:
Mouse
Kategorie:
Antikörper
Applikation:
ChIP, IHC, IP, WB
Immunogen:
Full length mouse CHOP/GADD153 from the N-terminal region
CHOP is a small nuclear protein that is capable of dimerizing with transcription factors C/EBP alpha and beta. Once dimerized, this complex inhibits the normal binding and function of C/EBP to classical binding sites. Inversely, the C/EBP-CHOP dimer gains binding activity to other non-classical C/EBP stress related targets. Under normal cellular conditions this protein is not expressed in detectable levels, but is highly unregulated during times of cellular/ER stress. Examples of CHOP inducing stress include: treatment with tunicamycin, nutrient starvation and reducing agents that interfere with the calcium flux across the ER membrane. Applications: Suitable for use in Immunocytochemistry, ChIP, Immunohistochemistry, Western Blot, Immunocytochemistry/Immunofluorescence, and Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunocytochemistry/Immunofluorescence: 1:100 Immunohistochemistry: 1:100 Immunohistochemistry (paraffin): 1:100 Immunoprecipitation: 1:10-1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
