Recombinant protein corresponding to aa30-2225 from rat Chondroitin Sulfate Proteoglycan. Species Sequence Homology: mouse.
NG2 is a high molecular weight, integral membrane chondroitin sufate proteoglycan. It is found on the surfaces of an unusual class of glial cells within the developing and mature central nervous system that have the properties of oligodendrocyte precursor cells (O-2A progenitor cells). NG2 is also found on the surfaces of chondroblasts, proliferating capillary endothelial cells, some human melanoma cell lines, and on leukemic blasts in childhood acute lymphoblastic leukemia. The NG2 proteoglycan is likely to play a role in regulation of cell motility, axon outgrowth and the cellular responses to platelet derived growth factor. Applications: Suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and ELISA. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Recommended Positive Control: Human melanoma, glioma and proliferating brain endothelial cells, rat B49 glial cell extracts or embryonic mouse brain tissue. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
