Pure Cicer arietinum lectin (CPA) from chick pea, FITC conjugated. Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms. Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose. Carbohydrate Specificity: Not yet determined. Inhibitory Carbohydrate: Not inhibited by simple sugars. Human IgM and bovine fetuin are potent inhibitors. Activity: Poor reactivity with normal human erythrocytes, requiring 7.5% BSA. Less than 1ug/ml will agglutinate papain treated cells. Chemical Used for Conjugation: FITC Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
Formulierung:
Supplied as a liquid in 0.01M phosphate, 0.15M sodium chloride, pH 7.2-7.4, 0.05% sodium azide. Labeled with FITC.
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