CLC3, Rat, Control Peptide (Clcn3, Chloride Channel) Control Peptide

Produktübersicht

• Artikelname: CLC3, Rat, Control Peptide (Clcn3, Chloride Channel) Control Peptide
• Artikelnummer: USB-C5837-10G
• Hersteller Artikelnummer: C5837-10G
• Alternativnummer: USB-C5837-10G-100
• Hersteller: US Biological
• Kategorie: Molekularbiologie
• Applikation: ELISA, WB

Produktbeschreibung

Control Peptide for C5837-10D (antiserum) and C5837-10E (affinity purified antibody). Synthetic peptide consisting of 18aa sequence near the C-terminus of rat CLC-3. No significant sequence homology is detected with other CLCs or other proteins. There is high degree of sequence homology between CLC3, CLC4 and CLC5. Species Sequence Homology: Mouse, guinea pig, rat, human, rabbit and frog CLC-3: 100%. Chloride is a critical component of all living cells. Voltage-gated chloride channels regulate cellular traffic of chloride ion. The chloride channels (CIC or CLC) performs several functions including the regulation of cell volume, membrane potential stabilization, signal transduction, and transepithelial transport. Mutations in CIC genes have been linked with several human diseases including myotonias (Thomsens disease), cystic fibrosis, Bartters syndrome type III, Dents disease, and X-linked recessive nephrolithiasis. In mammals, CLC proteins form a superfamily of at least 9 different genes (CLC1-7 also known as CLCN1-7 and CLK1-2 or CLCKa and CLCKb). Additional forms of these proteins are obtained by alternative splicing. All CLC proteins (~700-1000aa) are predicted to contain 10 (possibly 12) transmembrane domains. Except CLC-1 and CLC-K1/K2 that are specific for kidney, most other CLC are widely distributed in various tissues. Rat CLC-3 is 760aa membrane protein (human CLC-3 760 or 820aa, mouse CLC-3 760/818aa) (1). Alternatively spliced short forms of mouse and human CLC-3 have also been found. CLC-3 is abundant in brain (olfactory, hippocampus, and cerebellum) and moderate levels are found in the lung, kidney, and adrenals. CLC-3 has been implicated in neuronal cell function through regulation of membrane excitability by protein kinase C. It could also help neuronal cells to establish short-term memory. Applications: Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested. Recommended Dilution: Antibody Blocking: 5-10ug per 1ul C5837-10D (antiserum) or 1ug C5837-10E (affinity purified antibody). ELISA: 1:10,000-1:50,000. Control peptide can be used to coat ELISA plates at 1ug/ml. Optimal dilutions to be determined by the researcher. Control Peptide: C5837-10G: CLC3, Rat, Control Peptide (Clcn3, Chloride Channel) Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Reinheit: Highly purified
• Formulierung: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.