| For isolation of Clostridium difficile. Clostridium Difficile Agar is a formula recommended by George et al. for isolation of C. difficile. The medium contains fructose which enhances the recovery and growth of C. difficile. The addition of the antibiotics, cefoxitin and cycloserine, inhibit the growth of Enterobacteriaceae, Enterococcus faecalis, Staphylococci, Gram negative non-sporulating anaerobic bacilli, and Clostridia sp. but not C. difficile. Supplementing the medium with horse blood and egg yolk further improves the recovery of C. difficile. Microbiological Analysis: Bacteroides fragilis ATCC 8482: Inhibited Clostridium difficile ATCC 9689: Good Growth Clostridium perfringens ATCC 13124: Inhibited Staphylococcus aureus ATCC 25923: Inhibited *Results were obtained from type cultures after incubation at 35C in anaerobic conditions and observed at 48-72 hours. Media was prepared with 7% horse blood, 0.5g/L Clycloserine and 0.016g/L Cefoxitin Appearance: Light beige, homogeneous, free flowing powder Solubility: Light amber, clear to slightly hazy pH: 7.40.2 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |