| Clostripain (Endoproteinase-Arg-C) is a two chain proteinase associated with collagenase and isolated from Clostridium histolyticum. It is highly specific for the carboxyl peptide bond of arginine. Clostripain has a sulfhydryl requirement, it is activated by dithiothreitol, cysteine, or other sulfhydryl containing reagents. The presence of calcium ions is essential. The enzyme is inhibited by oxidizing agents and sulfhydryl reactants and by Co2+, Cu2+, Cd2+, and heavy metal ions. Citrate, borate, and Tris anions are less inhibitory. Activity: 50 units/mg dry weight Absorbance (280nm 1mg/ml): As Reported Protein: As Reported Unit Definition: 1 unit hydrolyzes 1umole of N-benzoyl-L-arginine ethyl ester (BAEE) per minute at 25C, pH 7.6, in the presence of 2.5mM dithiothreitol Quality Control: SDS-PAGE Storage and Stability: Lyophilized powder may be stored at 4C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
