Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600A in length and 15A in diameter and 300,000 molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched. FITC-labeled bovine type II is an excellent substrate for examining collagenase activity. This labeled substrate is highly purified and telo-peptide free and is supplied lyophilized or in solution. To minimize background levels in collagenase assays, FITC-labeled collagen has been enzymatically pre-treated and further purified by ion-exchange chromatography. FITC-labeled collagen can also be used as a substrate for cell culture. Collagen degradation products in the culture supernatants can be directly determined by measured the flourescence at 520nm (Emission)/490 nm (Excitation). Molar Ratio: ~1:1-Collagen: FITC Solubility: Soluble in acidic buffer (maximum 4mg/ml), but difficult to dissolve in neutral buffer. In order to dissolve in neutral buffer, dissolve in 0.01M acetic acid at 4mg/ml, and then add into neutral buffer (final sodium chloride concentration should be 0.15-0.2M). This does not form stable gel even in neutral conditions. Applications: Suitable for use in assaying mammalian collagenase activity. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 6 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molekulargewicht:
300
Reinheit:
99% by SDS-PAGE
Formulierung:
Supplied as a liquid in 0.01M acetic acid. Labeled with Fluorescein isothiocyanate (FITC).
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