Collagens are highly conserved throughout evolution and are characterised by an uninterrupted Glycine X Y triplet repeat that is a necessary part of the triple helical structure. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for Platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Source: Placental villi Applications: Suitable for use as Type IV collagen standard, antigen for antibody production, coating material for cell culture studies, and formation of collagen gels. Recommended Dilution: Optimal dilutions to be determined by the researcher. Inactivation: Pepsin digestion and storage in 500mM Acetic acid Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Reinheit:
Controlled and limited pepsin digestion, followed by selective salt precipitation. > 90% as judged by SDS-PAGE analysis.
Formulierung:
Supplied as a solution of collagen in 500mM acetic acid (C = 0.5mg/ml). The collagen may be transferred into physiological buffers by dialysis at 2-8C.
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