Collagen is the main structural protein in the extracellular space and is the most abundant protein in the ECM. Collagens are divided into two classes - fibril (types I, II, III, V) and non-fibril (types IV, VI). Type V collagen is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen mutations are associated with Ehlers-Danlos syndrome. Type V collagen is broadly expressed as a two alpha1(V) chains and one alpha2(V) chain heterotrimer but also as a alpha1(V), alpha2(V), and alpha3(V) heterotrimer in pancreatic islets, adipose tissue, and skeletal muscle. Applications: Suitable for use in ELISA and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Reinheit:
Controlled and limited pepsin digestion, followed by selective salt precipitation. 90% as judged by SDS-PAGE analysis.
Formulierung:
Supplied as a liquid in 500mM acetic acid.
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