| Collagenases degrade native helical collagen fibrils.The substrate, collagen, is the major fibrous component of animal extracellular connective tissue: skin, tendon, blood vessels, bone, etc. Suitable for use in cell dissociation procedures. Collagenase Nomenclature: Type 1-Containing average amounts of assayed activities (collagenase, caseinase, clostripain and tryptic activities). It is generally recommended for fat, adrenal, and liver cells. Type 2-Containing greater clostripain activity. It is generally used for heart, bone, muscle, thyroid, cartilage, and liver cells. Type 3-Containing low proteolytic activity. It is usually used for mammary and fetal cells. Type 4-Containing low tryptic activity. It is commonly used for islets and other applications where receptor integrity is crucial. Absorbance: As reported Activity: 500 units/mg dry weight (dw) 50 units/mg Caseinase (dw) 2.0 units/mg Clostripain (dw) 0.25 units/mg Tryptic (dw) Unit Definition: 1 unit releases 1umole of L-leucine equivalents from collagen in 5 hours at 37C, pH 7.5. Units per Vial (10Ku): 10,000 units/vial Endotoxin EU/mg dw: As Reported Quality Control: SDS-PAGE Storage and Stability: Collagenase is stable for 6 months at 4C. |
