Collagenase, Type II, Sterile Filtered, C. histolyticum, CAS [[9001-12-1]]
Artikelnummer:
USB-C7511-40
Hersteller Artikelnummer:
C7511-40
Alternativnummer:
USB-C7511-40-50
Hersteller:
US Biological
Kategorie:
Molekularbiologie
Collagenases degrade native helical collagen fibrils. The substrate, collagen, is the major fibrous component of animal extracellular connective tissue: skin, tendon, blood vessels, bone, etc. Suitable for use in cell dissociation procedures. Collagenase is prepared to contain higher clostripain activity levels. Suitable for use in heart, bone, thyroid, salivary and liver primary cell isolation. Collagenase nomenclature: Type 1: Containing average amounts of assayed activities (collagenase, caseinase, clostripain and tryptic activities). It is generally recommended for fat, adrenal, and liver cells. Type 2: Containing greater clostripain activity. It is generally used for heart, bone, muscle, thyroid, cartilage, and liver cells. Type 3: Containing low proteolytic activity. It is usually used for mammary and fetal cells. Type 4: Containing low tryptic activity. It is commonly used for islets and other applications where receptor integrity is crucial. Source: Clostridium histolyticum Activity (dw): 125u/mg dry weight Units/Vial: 6250u/vial Units/mg Caseinase (dw): 200u/mg dry weight Units/mg Clostripain (dw): 3.5u/mg dry weight Units/mg Tryptic (dw): 3.5u/mg dry weight Unit Definition: 1 unit releases 1umole of L-leucine equivalents from collagen in 5 hours at 37C, pH 7.5 Absorbance 280nm: As Reported Colony Count: 1cfu/ml at 37C, 2ml recon., 48 hrs. Quality Control: SDS-PAGE Sterility: Filtered through a 0.22 micron membrane Form: Supplied as a dialyzed, lyophilized powder Storage and Stability: Store lyophilized powder desiccated at 4C. Storage at -20C (desiccated) is acceptable.
Formulierung:
Supplied as a dialyzed, lyophilized powder.
CAS Nummer:
[9001-12-1]
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