Collectins constitute a family of C-type lectins that recognize molecular patterns expressed on pathogens. Members of this glycoprotein family contain a N-terminal domain, a collagen-like domain, a neck region, and a C-terminal carbohydrate recognition domain (CRD). Collectins are typically secreted molecules, although CL-P1 is membrane bound and CL-L1 is found in the cytoplasm. Collectin kidney 1 (CL-K1), also known as collectin subfamily member 11 (COLEC11), is a 37kD collectin that circulates in the serum. It associates into disulfide-linked oligomers and preferentially interacts with fucose residues in a calcium-dependent manner. Mature mouse CL-K1 shares 94% and 98% amino acid sequence identity with human and rat CL-K1, respectively. Within the CRD, mouse CL-K1 shares 52% aa sequence identity with CL-L1 and 23%-32% aa sequence identity with collectins CL-P1, MBL, SP-A, and SP-D. DNA sequence encoding mouse CL-K1 (Gln27-Leu272), fused to a 6X His-Tag at the C-terminus, expressed in a mouse myeloma cell line, NS0. Activity: Measured by its ability to bind Alpha-L-Fucose. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: As a result of glycosylation the recombinant mouse CL-K1 migrates as an ~33-38kD protein in SDS-PAGE under reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
