Complement Component C2 (C2) is a serine protease that is part of the classical pathway of the complement system. The classical complement pathway plays a major role in innate immunity against infection. This pathway is triggered by C1, a multimolecular complex composed of the recognition protein C1q and two serine proteases, C1r and C1s. After proteolytic activation by C1, the single chain form of C2 aa19-760 becomes two chains, which are referred to as C2A and C2B. C2A aa251-760 consists of a vWF domain aa260-457 and a serine protease domain aa480-760. After the activation by C1, C2A combines with complement factor 4B to generate the C3 or C5 convertase. The full length mouse C2 was expressed, and the purified protein consists of a mixture of single chain and C2A form based on the N-terminal sequencing data. Recombinant corresponding to aa1-760 from mouse Complement Component C2, fused to 6-His tag at C-terminal, expressed in mouse myeloma cell line, NS0. Molecular Weight: ~58kD, 84kD Biological Activity: Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy- Gly-Arg-ThioBenzyl ester (Z-GR-SBzl), in the presence of 5,5Dithio-bis (2-nitrobenzoic acid) (DTNB). Specific Activity: >200pmol/min/ug Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
