Recombinant corresponding to mouse Complement C5a R1 expressed in NSO cells (P30993).
C5a R1, also known as C5a ligand and CD88, is a 7 transmembrane protein expressed on myeloid, endothelial, epithelial, and smooth muscle cells. C5a R1 binds the activated complement anaphylatoxin C5a. In established allergic environments, this triggers neutrophil and eosinophil chemotaxis and the release of proinflammatory mediators. In contrast, C5a R1/C5a interactions are protective during allergen sensitization. Mouse C5a R1 shares 66-77aa sequence identity with human and rat C5a R, respectively. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Hybridoma: Myeloma cells with B cells from Balb/c mice. Absorption: 620-650nm Emission: 660-670nm Storage and Stability: May be stored at 4C before opening. DO NOT FREEZE! Stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. APC is light sensitive.
