During complement activation the circulating complement compound C6 is bound by the C3b5b complex to form the first stage of the membrane attack complex. The formed C5b6 complex binds to C7 where after C8 binds, followed by binding of a variable amount of C9 molecules. The complement factor C6 is an acute-phase reactant for which a CCAAT enhancer binding site essential is. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:10 Immunohistochemistry (Frozen): 1:10 Note: Dilute C7850-35B in TBS, not in PBS. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Reinheit:
Purified by Protein A affinity chromatography.
Formulierung:
Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
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