Factor P is a 53kD glycosylated protein present in blood serum. Factor P binds to microbial surfaces and binds and stabilizes the complement component 3- and complement component 5-convertase enzyme complexes. C3b then acts as an opsonin or initiates the complement pathway again creating a positive feedback loop which acts only on the bacterial cell, leaving the host cells unharmed. Deficiency of factor P makes the host more susceptible to bacterial infections, particularly to Neisseria species and meningococcal infections. Applications: Suitable for use in ELISA, Functional Assays, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Recommended Positive Control: Kidney from post strepoccal glomerulonephritis patients Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
