Connexin 32 (CX32, CB1, Gap Junction Beta1 Protein, Gap Junction Liver Protein) (HRP), Clone: [2Q252 M12.13], Mouse, Monoclonal
Artikelnummer:
USB-C7854-02-HRP
Hersteller Artikelnummer:
C7854-02-HRP
Alternativnummer:
USB-C7854-02-HRP-100
Hersteller:
US Biological
Wirt:
Mouse
Kategorie:
Antikörper
Applikation:
IHC, WB
Immunogen:
Directed against an epitope located between residues 95-125 in the central cytoplasmic loop of rat Cx32.
Applications: Suitable for use in Immunohistochemistry (lightly formaldehyde fixed tissues) and Western Blot. Other applications have not been tested. Recommended Dilution: Western blot: Detects a 27kD Connexin 32 polypeptide in blots of rat hepatocyte gap junctions and an additional 47kD band corresponding to aggregates. Optimal dilution to be determined by the researcher. Hybridoma: Produced by the immunization of BALB/c mice with isdolated rat junctional complexes. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
