Connexin 43, Mouse (CX43, Gap Junction Alpha 1 Protein, CXA-1) Control Peptide

Produktübersicht

• Artikelname: Connexin 43, Mouse (CX43, Gap Junction Alpha 1 Protein, CXA-1) Control Peptide
• Artikelnummer: USB-C7857-07A
• Hersteller Artikelnummer: C7857-07A
• Alternativnummer: USB-C7857-07A-100
• Hersteller: US Biological
• Kategorie: Molekularbiologie
• Applikation: ELISA

Produktbeschreibung

Control Peptide for C7857-07 (antiserum) and C7857-07B (affinity purified antibody). Mouse connexin 43 is a 382 amino acid gap junction protein with a predicted MW ~43kD. It is prominently expressed in heart. Gap junctions are composed of transmembrane channels that link the cytoplasm of neighboring cells. They differ from other membrane channels since they exist between two cells. Gap junctions are relatively non-specific and allow passive diffusion of small molecules up to 1kD. The junctions exist in almost all vertebrate and non-vertebrates cells. It is believed that gap junction play an important for intercellular communications and affect growth and differentiation of cells. Absence of intercellular communication mediated by gap junction may lead to transformed or cancerous growth. Gap junctional channel is composed of a hemichannel (connexon) in the cell membrane of one cell joined in mirror symmetry with a connexon in the opposing cell. Each connexon is an oligomer of six protein subunits that define the axial aqueous pore. Molecular cloning studies have identified a family of at least 12 highly related Connexins that are designated according to MW, Cx26-50. Hydropathy analyses of Cx sequences predicts 4 transmembrane domains: 2 extracellular (EC) and 3 cytoplasmic (CP) domains. The EC, TM, and N-terminal CP domains are well conserved among family members, while Central and C-terminal domains are highly variable in both sequence and size. The N and C-termini are predicted to be cytoplasmic. Applications: Suitable for use in ELISA, Antibody Blocking. Not suitable for use in Immunoblot. Other applications not tested. Recommended Dilutions: ELISA: 50-100ng control peptide per well. Antibody Blocking: 5-10ug per 1ul C7857-07 (antiserum) or per 1ug C7857-07B (affinity purified antibody). Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Reinheit: Highly purified
• Formulierung: Supplied as a liquid in PBS, pH 7.5, 0.09% sodium azide.