CREB (cAMP Response Element Binding Protein), Rabbit

Produktübersicht

• Artikelname: CREB (cAMP Response Element Binding Protein), Rabbit
• Artikelnummer: USB-C7915
• Hersteller Artikelnummer: C7915
• Alternativnummer: USB-C7915-200
• Hersteller: US Biological
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IP, WB
• Immunogen: KLH-conjugated, synthetic peptide (C-SGAENQQSGDAAVTEAENQQ) corresponding to amino acids 5-24 of human CREB. The immunizing sequence is identical in pig, shares 19 of 20 residues with mouse and bovine and 18 of 20 residues with rat.

Produktbeschreibung

Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-2mg/ml detects CREB from a HeLa nuclear lysate. Representative blot from a previous lot: HeLa nuclear lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-CREB ( 0.5ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Positive Antigen Control: HeLa nuclear extract. Add 2.5ul of 2- mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels. Immunoprecipitation: 5mg (will immunoprecipitated CREB from 500mg of HeLa nuclear extract). Optimal dilution determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Produkteigenschaften

• NCBI: 604391
• UniProt: P16220
• Reinheit: Purified by Protein A affinity chromatography.
• Formulierung: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.09% sodium azide, 30% glycerol