| Cysteine rich motor neuron 1 (CRIM1) is a type I transmembrane glycoprotein of the chordin-like cysteine-rich repeat (CRR) family of BMP inhibitors. The ~130kD, 1036aa CRIM1 contains a 34aa signal sequence, a 905aa extracellular domain (ECD), a 21aa transmembrane domain and a 76aa cytoplasmic domain. The ECD includes an N-terminal IGF-binding protein-like motif and six chordin-like von Willebrand C-type CRRs. The ECD can be released from the cell , presumably by proteolytic processing. Human CRIM1 ECD shows 88%, 88%, 91%, 86%, 87%, 83% and 72% aa identity with mouse, rat , dog, cow, opossum, chick and zebrafish CRIM1 ECD, respectively. CRIM1 can interact with TGF-beta family ligands, including BMPs 2, 4 and 7, via its CRR domains. It binds BMPs intracellularly and antagonizes them by lowering their expression, processing and secretion. CRIM1 is expressed in the developing spinal cord in the floor plate and developing motor neurons. It is also expressed by perivascular smooth muscle cells and aligns at points of cell-cell contact during endothelial cell capillary formation. Endothelial cell expression in vitro appears to be specific to cells that are adherent and growing. CRIM1 is also expressed in a spatially and temporally restricted manner in the developing lens, limbs, kidney, teeth and testis. Studies where CRIM1 expression is manipulated in developing mouse, chick and zebrafish support its involvement in regulation of vascular and somitic development and organogenesis. Source: Murine myeloma cell line, NS0-derived. Leu35-Ser939, with a C-terminal 6-His tag. Molecular Weight: 125-140kD (SDA-PAGE, reducing conditions) Biological Activity: Measured by the ability of the immobilized protein to support the adhesion of NIH/3T3 mouse embryonic fibroblasts. CRIM1 immobilized at 3ug/ml, 100ul/well, will mediate >50% NIH3T3 cell adhesion (added at 1x10e5 cells/well). Endotoxin: 1EU/ug (LAL) Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
