Crystallin, alpha B (alpha B Crystallin Protein, Alpha(B) Crystallin, Alpha Crystallin B Chain, CRYAB, Crystallin alpha 2, CRYA2, CTPP2, Heat Shock 20kD Like Protein, Heat Shock Protein beta 5, HSPB5, NY-REN 27 Antigen, Renal Carc
Artikelnummer:
USB-C7944-74K
Hersteller Artikelnummer:
C7944-74K
Alternativnummer:
USB-C7944-74K-200
Hersteller:
US Biological
Wirt:
Mouse
Kategorie:
Antikörper
Applikation:
IF, WB
Immunogen:
Native purified human CRYAB.
The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alpha-crystallins possess structural and functional similarities with Hsp25 and Hsp27 (1). Mammalian lens cystallins are divided into alpha, beta and gamma families. Alpha and beta families are further divided into acidic and basic groups (Alpha-A and Alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its phosphorylated state and may serve a structural control function and play a role in protein maintenance (2). In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA (3, 4, 5, 6, and 7). Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases (8). Specifically, alpha-B cystallin is found in many cells and organs outside the lens, and alpha B is overexpressed in several neurological disorders and in cell lines under stress conditions (9). Applications: Suitable for use in Immunocytochemistry/Immunofluorescence and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Klonalität:
Monoclonal
Klon-Bezeichnung:
[1A7.D5]
Isotyp:
IgG1
Reinheit:
Purified by Protein G affinity chromatography.
Formulierung:
Supplied as a liquid in PBS, pH 7.2, 0.09% azide, 50% glycerol.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
