Cytochrome c BioAssay(TM) ELISA Kit

Produktübersicht

• Artikelname: Cytochrome c BioAssay(TM) ELISA Kit
• Artikelnummer: USB-C9094-90
• Hersteller Artikelnummer: C9094-90
• Alternativnummer: USB-C9094-90-1
• Hersteller: US Biological
• Kategorie: Kits/Assays
• Applikation: ELISA

Produktbeschreibung

Principle of the Assay: The Cytochrome C ELISA kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA). A monoclonal antibody specific for Cytochrome c has been coated onto the wells of the microtiter strips provided. Samples, including a standard containing Cytochrome c, control specimens and unknowns, are pipetted into these wells. During the first incubation, the Cytochrome c antigen binds to the immobilized (capture) antibody. After washing, a biotinylated secondary antibody specific for Cytochrome c is added to the wells. During the second incubation, this antibody serves as a detection antibody by binding to the immobilized Cytochrome c protein captured during the first incubation. After removal of excess detection antibody, a Streptavidin-Peroxidase (enzyme) is added. This binds to the biotinylated detection antibody to complete the four-member sandwich. After a third incubation and washing to remove all the excess Streptavidin-Peroxidase, a substrate solution is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of Cytochrome c present in the original specimen. Kit Components: C9094-90A: Microwell Plate, 1x96 wells Supplied as removable strips coated with mouse monoclonal antibody to Cytochrome c. C9094-90B*: Standard, 2 x1vial Supplied as 10ng of lyophilized powder. C9094-90C: Standard Diluent Buffer, 1x25ml Supplied as a liquid, 0.1% sodum azide, red dye**. C9094-90D: Pab (Biotin), 1x11ml Biotin-labeled anti-Cytochrome c antibody. Supplied as a liquid, 0.1% sodium azide, blue dye**. C9094-90E: Streptavidin (HRP) 100X, 1x125ul Supplied as a liquid, 3.3mM thymol, 50% glycerol. C9094-90F: HRP Diluent, 1x25ml Supplied as a liquid, 3.3mM thymol, yellow dye**. C9094-90G: Wash Buffer 25X, 1x100ml C9094-90H: TMB Substrate, 1x25ml C9094-90J: Stop Solution, 1x25ml Storage and Stability: *Store reconstituted C9094-90B at -70C. Store all other components at 4C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Analytical Sensitivity: <0.156ng/ml of Cytochrome c Specificity: Detects human, rat and mouse forms of the Cytochrome c protein.