Keratins are a class of fibrous proteins or scleroproteins important both as structural proteins and as keys to the study of protein conformation. The family represents the principal constituent of epidermis, hair, nails, horny tissues, and the organic matrix of tooth enamel. Two major conformational groups have been characterized, alpha-keratin, whose peptide backbone forms an alpha-helix, and beta-keratin, whose backbone forms a zig-zag or pleated sheet structure. Recombinant protein corresponding to Ser2-His430 from human Cytokeratin 18, fused to 6X His-Tag at N-terminal, expressed in E. coli. Swiss/Uniprot: P05783 Molecular Weight: ~49kD Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant KRT18 at 1ug/mL (100uL/well) can bind Human KRT8 with EC50 at 2.4ug/ml Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
