Cytokeratin 7 (Cytokeratin-7, CK 7, CK7, CK-7, Keratin 55k Type II Cytoskeletal, Keratin 7, Keratin-7, K7, KRT7, Keratin Simple Epithelial Type 1 K7, Keratin Type II Cytoskeletal 7, K2C7, MGC3625, MGC129731, Sarcolectin, SCL, Type
Cytokeratin 7 (Cytokeratin-7, CK 7, CK7, CK-7, Keratin 55k Type II Cytoskeletal, Keratin 7, Keratin-7, K7, KRT7, Keratin Simple Epithelial Type 1 K7, Keratin Type II Cytoskeletal 7, K2C7, MGC3625, MGC129731, Sarcolectin, SCL, Type
Cytokeratin 7 (Cytokeratin-7, CK 7, CK7, CK-7, Keratin 55k Type II Cytoskeletal, Keratin 7, Keratin-7, K7, KRT7, Keratin Simple Epithelial Type 1 K7, Keratin Type II Cytoskeletal 7, K2C7, MGC3625, MGC129731, Sarcolectin, SCL, Type
Artikelnummer:
USB-C9097-40D1
Hersteller Artikelnummer:
C9097-40D1
Alternativnummer:
USB-C9097-40D1-100
Hersteller:
US Biological
Wirt:
Mouse
Kategorie:
Antikörper
Applikation:
FC, IHC, WB
Immunogen:
Cytoskeletal proteins from cultured HeLa cells.
Cytokeratin 7 has been found in columnar and glandular epithelium of the lung, cervix, breast, bile ducts and larger collecting ducts of the kidney. Occasionally staining of blood vessel cell walls, particularly endothelial cells, may be observed. The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants, however, not all variants have been fully described. Applications: Suitable for use in Western Blot, Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: 10ul per test Immunocytochemistry: 1:10 using isolated EVT cells fixed with 0.5% PFA at 4C for 15 minutes followed by acetone at -20C for 2 minutes. Immunohistochemistry (Paraffin): Protease treatment of paraffin sections is required. Immunohistochemistry (Frozen): 1:10 used with placental tissue (7um) fixed with acetone for 1 hour. Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
