ELISA Kit for Antibody to Cytomegalovirus (CMV) is an in vitro enzyme immunoassay for the detection of Anti-CMV antibodies IgM in human serum or plasma. It is for diagnosis of early infection and epidemic survey. Principle: This kit uses capture ELISA method to detect anti-CMV antibodies IgM in serum or plasma. The anti-human IgM (mu chain) antibody is coated on the multi-wells plate. The HRP conjugated recombinant CMV antigen serves as tracer. TMB is substrate for HRP. The enzyme reaction with substrate TMB produces a color change, and the intensity of the absorbance at 450 nm indicates the presence or absence of Anti-CMV antibodies IgM in the sample. The test is specific, sensitive, reproducible and easy to operate. It is for blood screen of CMV infection. Kit Components: 1. Antibody Coated Microwell Plate, 1x96 wells 2. Enzyme Conjugant 3. Negative Control Serum 4. Positive Control Serum 5. Concentrated Wash Buffer (20x) 6. Substrate A 7. Substrate B 8. Stop Solution Storage and Stabilty: Store components at 4C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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