| Dectin-1, also known as CLEC7A and the beta-glucan receptor, is a 43kD type II transmembrane C-type lectin that functions in the innate immune response to fungal pathogens. Although Dectin-1 resembles other CLEC molecules structurally, it binds ligands in a calcium-independent manner. Mature mouse Dectin-1 is a 244 aa glycoprotein that consists of a short ITAM-containing cytoplasmic tail, a transmembrane segment, and a stalk and carbohydrate recognition domain (CRD) in the extracellular domain. The CRD of mouse Dectin-1 shares 61%, 60%, and 87% aa sequence identity with that of bovine, human, and rat Dectin-1, respectively. It shares 25%-34% aa sequence identity with the CRD of other subgroup members CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL. Mouse Dectin-1 is alternately spliced, generating a variant that lacks the stalk region. Mouse Dectin-1 is expressed on monocytes, macrophages, and neutrophils, and on some populations of dendritic cells and T cells. It is upregulated on macrophages by GM-CSF, IL-4, or IL-13 and downregulated by dexamethasone, IL-10, or LPS. The CRD selectively binds beta- glucan polymers, a major component of yeast and mycobacterial cell walls. Yeast beta-glucan is accessible to Dectin-1 only at sites of cell budding, and Dectin-1 does not recognize the filamentous form of yeast. Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast. It colocalizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling. Dectin-1 interaction with the tetraspanin CD37 increases its stability on the cell membrane and inhibits ligand-induced signaling. Genetic knockout of Dectin-1 in mice increases their susceptibility to pathogenic infection. Recombinant protein corresponding to Phe69-Leu244 with an N-Terminal 10X His-tag from mouse Dectin-1, expressed in NS0 cells. Uniprot/Accession: Q6QLQ4. Activity: Measured by its ability to bind biotinylated laminarin (1, 3-beta-glucan) with an estimated KD <6nM. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
